Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Silencing of long non-coding RNA SNHG15 suppresses proliferation, migration and invasion of pancreatic cancer cells by regulating the microRNA-345-5p/RAB27B axis

doi: 10.3892/etm.2021.10708

Figure Lengend Snippet: Knockdown of long non-coding RNA SNHG15 inhibits proliferation, migration and invasion of pancreatic cancer cells. (A) Relative expression of SNHG15 in BXPC-3 and PANC-1 cells after transfection with si-SNHG15-1, si-SNHG15-2 and si-NC was detected by reverse transcription-quantitative polymerase chain reaction. **P<0.01, vs. si-NC. (B) Cell viability in BXPC-3 and PANC-1 cells was detected by 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide assay. *P<0.05, **P<0.01, vs. si-NC. (C) Wound-healing rate of BXPC-3 and PANC-1 cells was detected by wound-healing assay. **P<0.01, vs. si-NC. (D) Number of invading cells was detected by Transwell assay. **P<0.01, vs. si-NC. (E) The growth of tumour xenograft in mice injected with PANC-1 cells transfected with Lv-si-SNHG15-1 or Lv-si-NC. **P<0.01, vs. Lv-si-NC. (F) The expression levels of SNHG15, miR-345-5p and RAB27B in tumour xenograft after injection with Lv-si-SNHG15-1 or Lv-si-NC. **P<0.01, vs. Lv-si-NC. si-, small interfering RNA-; NC, negative control; miR, microRNA; Lv, lentivirus vector.

Article Snippet: Small interfering RNAs (siRNAs) against SNHG15 (si-SNHG15-1 and si-SNHG15-2), siRNA negative control (si-NC), miR-NC, miR-345-5p mimics, inhibitor NC, miR-345-5p inhibitor, pcDNA-RAB27B and empty vector (pcDNA-NC) were purchased from Guangzhou RiboBio Co., Ltd..

Techniques: Knockdown, Migration, Expressing, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Wound Healing Assay, Transwell Assay, Injection, Small Interfering RNA, Negative Control, Plasmid Preparation

Journal: Experimental and Therapeutic Medicine

Article Title: Silencing of long non-coding RNA SNHG15 suppresses proliferation, migration and invasion of pancreatic cancer cells by regulating the microRNA-345-5p/RAB27B axis

doi: 10.3892/etm.2021.10708

Figure Lengend Snippet: miR-345-5p is the target gene of SNHG15. (A) The binding sequence between SNHG15 and miR-345-5p was predicted by StarBase ver2.0. (B) Relative expression of miR-345-5p was detected by RT-qPCR. **P<0.01, vs. si-NC. (C) The interaction between SNHG15 and miR-345-5p in BXPC-3 and PANC-1 cells was validated by dual-luciferase reporter assay. **P<0.01, vs. miR-NC. (D) Relative expression of miR-345-5p was detected by RT-qPCR in tumour tissues and adjacent non-cancerous tissues. **P<0.001, vs. adjacent non-cancerous tissues. (E) Correlation analysis of SNHG15 and miR-345-5p in tumour tissues. (F) Relative expression of miR-345-5p in HPDE6, BXPC-3 and PANC-1 cells detected by RT-qPCR. **P<0.01, vs. HPDE6. miR, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si-, small interfering RNA-; NC, negative control; WT, wild type; MUT, mutant.

Article Snippet: Small interfering RNAs (siRNAs) against SNHG15 (si-SNHG15-1 and si-SNHG15-2), siRNA negative control (si-NC), miR-NC, miR-345-5p mimics, inhibitor NC, miR-345-5p inhibitor, pcDNA-RAB27B and empty vector (pcDNA-NC) were purchased from Guangzhou RiboBio Co., Ltd..

Techniques: Binding Assay, Sequencing, Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Small Interfering RNA, Negative Control, Mutagenesis

Journal: Experimental and Therapeutic Medicine

Article Title: Silencing of long non-coding RNA SNHG15 suppresses proliferation, migration and invasion of pancreatic cancer cells by regulating the microRNA-345-5p/RAB27B axis

doi: 10.3892/etm.2021.10708

Figure Lengend Snippet: Long non-coding RNA SNHG15 regulates cell proliferation, migration and invasion through miR-345-5p/RAB27B axis. (A) Protein expression of RAB27B was detected by western blotting. **P<0.01 vs. si-NC. ##P<0.01 vs. miR-NC. &&P<0.01 vs. pcDNA-NC. (B) Cell viability was determined by 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide assay in PANC-1 cells. *P<0.05, **P<0.01, vs. si-NC. #P<0.05, vs. si-SNHG15-1. (C) Cell migration was determined by wound-healing assay. **P<0.01, vs. si-NC. ##P<0.01, vs. si-SNHG15-1. (D) Cell invasion in PANC-1 cells was detected by Transwell assay. **P<0.01, vs. si-NC. ##P<0.01, vs. si-SNHG15-1. miR, microRNA; NC, negative control; si-, small interfering RNA-.

Article Snippet: Small interfering RNAs (siRNAs) against SNHG15 (si-SNHG15-1 and si-SNHG15-2), siRNA negative control (si-NC), miR-NC, miR-345-5p mimics, inhibitor NC, miR-345-5p inhibitor, pcDNA-RAB27B and empty vector (pcDNA-NC) were purchased from Guangzhou RiboBio Co., Ltd..

Techniques: Migration, Expressing, Western Blot, Wound Healing Assay, Transwell Assay, Negative Control, Small Interfering RNA

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p overexpression inhibits the proliferation, migration and invasion of AMC-HN-8 cells. (A) miR-195-5p overexpression efficiency was detected by RT-qPCR. (B) Cell proliferation in each group following transfection was detected by the Cell Counting Kit-8 assay. (C and D) Cell migration in each group following transfection was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection was detected by Transwell assays. Magnification, x100. The levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9 were respectively examined by (G) western blot analysis and (H) RT-qPCR assay. ** P<0.01; and *** P<0.001. miR, microRNA; RT-qPCR, reverse transcription-quantitative; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Over Expression, Migration, Quantitative RT-PCR, Transfection, Cell Counting, Western Blot, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: E2F3 is a direct target gene of miR-195-5p. (A) TargetScan and ENCORI databases were used to predict the binding sequence between miR-195-5p and E2F3. (B) Dual-luciferase reporter assay was used to analyze the association between miR-195-5p and E2F3. (C) RT-qPCR was used to evaluate the effect of miR-195-5p overexpression on E2F3 expression. (D) RT-qPCR was used to evaluate the expression of E2F3 in AMC-HC-8 cells. * P<0.05; ** P<0.01; and *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; WT, wild type; MUT mutant.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Binding Assay, Sequencing, Luciferase, Reporter Assay, Quantitative RT-PCR, Over Expression, Expressing, Real-time Polymerase Chain Reaction, Negative Control, Mutagenesis

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control